Journal articles
HACD1 , a regulator of membrane composition and fluidity, promotes myoblast fusion and skeletal muscle growth
Jordan Blondelle
1
Yusuke Ohno
2
Vincent Gache
1
Stéphane Guyot
3
Sébastien Storck
4
Nicolas Blanchard-Gutton
1
Inès Barthélémy
1
Gemma Walmsley
1
Anaëlle Rahier
1
Stéphanie Gadin
1
Marie Maurer
1
Laurent Guillaud
1
Alexandre Prola
1
Arnaud Ferry
5
Geneviève Aubin-Houzelstein
1
Jean Demarquoy
6
Frédéric Relaix
1
Richard Piercy
7
Stéphane Blot
1
Akio Kihara
2
Laurent Tiret
1
Fanny Pilot-Storck
1
3
PMB - Procédés Microbiologiques et Biotechnologiques
PAM - Procédés Alimentaires et Microbiologiques, PAM - Procédés Alimentaires et Microbiologiques [Dijon]
Jordan Blondelle 1
Author
Yusuke Ohno 2
Author
Vincent Gache 1
Author
Stéphane Guyot 3
Author IdHAL : stephane-guyot ResearcherId : http://www.researcherid.com/rid/A-3482-2012 ORCID : https://orcid.org/0000-0002-2316-234X
Sébastien Storck 4
Author
Nicolas Blanchard-Gutton 1
Author
Inès Barthélémy 1
Author
Gemma Walmsley 1
Author
Alexandre Prola 1
Author
Arnaud Ferry 5
Author
Geneviève Aubin-Houzelstein 1
Author IdHAL : genevieve-aubin-houzelstein
Jean Demarquoy 6
Author
Frédéric Relaix 1
Author IdHAL : fredericrelaix
Richard Piercy 7
Author
Stéphane Blot 1
Author IdHAL : sblot ResearcherId : http://www.researcherid.com/rid/K-7573-2019
Akio Kihara 2
Author
Laurent Tiret 1
Author IdHAL : laurenttiret ResearcherId : http://www.researcherid.com/rid/K-3877-2017 ORCID : https://orcid.org/0000-0001-8573-8335
Fanny Pilot-Storck 1
Author
1
IMRB - Institut Mondor de Recherche Biomédicale (8 rue du Général Sarrail 94010 Créteil - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U955 (101, rue de Tolbiac, 75013 Paris - France)
- IFR10 (France)
- UPEC UP12 - Université Paris-Est Créteil Val-de-Marne - Paris 12 : UMR955 (61 avenue du Général de Gaulle - 94010 Créteil cedex - France)
2
Hokkaido University [Sapporo, Japan] (5 Chome Kita 8 Jōnishi, Kita Ward, Sapporo, Hokkaido Prefecture 060-0808, Japon
- Japan)
3
PMB - Procédés Microbiologiques et Biotechnologiques (UMR-PAM - Batiment EPICURE - 1 Esplanade Erasme - 21000 Dijon - )
- PAM - Procédés Alimentaires et Microbiologiques (Université de Bourgogne - 1 esplanade Erasme - 21000 Dijon - France)
- UB - Université de Bourgogne (Maison de l'université - Esplanade Érasme - BP 27877 - 21078 Dijon cedex - France)
- AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement : UMR MA AGROSUP 2012.02.102 (26 Boulevard du Dr Petitjean - BP 87999 - 21079 Dijon cedex - France)
- PAM - Procédés Alimentaires et Microbiologiques [Dijon] (Université Bourgogne Franche-Comté - AgroSup Dijon - Batiment Epicure - 1 esplanade Erasme - F-21000 Dijon - France)
- UBFC - Université Bourgogne Franche-Comté [COMUE] (France)
- UB - Université de Bourgogne (Maison de l'université - Esplanade Érasme - BP 27877 - 21078 Dijon cedex - France)
- AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement : UMR-MA 2012.02.102 (26 Boulevard du Dr Petitjean - BP 87999 - 21079 Dijon cedex - France)
4
INEM - UM 111 (UMR 8253 / U1151) - Institut Necker Enfants-Malades (Bâtiment Leriche
14 rue Maria Helena Vieira Da Silva
75014 Paris - France)
- UPD5 - Université Paris Descartes - Paris 5 : UM 111 (12, rue de l'École de Médecine - 75270 Paris cedex 06 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1151 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR 8253 (France)
5
Thérapie des maladies du muscle strié (105 Bd de L'Hôpital 75013 Paris - France)
- UPMC - Université Pierre et Marie Curie - Paris 6 (4 place Jussieu - 75005 Paris - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U974 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR7215 (France)
6
UB - Université de Bourgogne (Maison de l'université - Esplanade Érasme - BP 27877 - 21078 Dijon cedex - France)
7
Comparative Neuromuscular Diseases Laboratory (London - United Kingdom)
- Royal Veterinary College (France)
Abstract : The reduced diameter of skeletal myofibres is a hallmark of several congenital myopathies, yet the underlying cellular and molecular mechanisms remain elusive. In this study, we investigate the role of HACD1/PTPLA, which is involved in the elongation of the very long chain fatty acids, in muscle fibre formation. In humans and dogs, HACD1 deficiency leads to a congenital myopathy with fibre size disproportion associated with a generalized muscle weakness. Through analysis of HACD1-deficient Labradors, Hacd1-knockout mice, and Hacd1-deficient myoblasts, we provide evidence that HACD1 promotes myoblast fusion during muscle development and regeneration. We further demonstrate that in normal differentiating myoblasts, expression of the catalytically active HACD1 isoform, which is encoded by a muscle-enriched splice variant, yields decreased lysophosphatidylcholine content, a potent inhibitor of myoblast fusion, and increased concentrations of ≥C18 and monounsaturated fatty acids of phospholipids. These lipid modifications correlate with a reduction in plasma membrane rigidity. In conclusion, we propose that fusion impairment constitutes a novel, non-exclusive pathological mechanism operating in congenital myopathies and reveal that HACD1 is a key regulator of a lipid-dependent muscle fibre growth mechanism.
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Journal articles
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https://hal-agrosup-dijon.archives-ouvertes.fr/hal-02290631
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Submitted on : Thursday, May 28, 2020 - 12:54:16 PM
Last modification on : Wednesday, October 27, 2021 - 4:00:37 PM
Contributor : Admin Agrosupdijon Connect in order to contact the contributor
Submitted on : Thursday, May 28, 2020 - 12:54:16 PM
Last modification on : Wednesday, October 27, 2021 - 4:00:37 PM
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- HAL Id : hal-02290631, version 1
- DOI : 10.1093/jmcb/mjv049
- PRODINRA : 360621
- PUBMED : 26160855
- WOS : 000363201800005
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SU-MEDECINE | AGROSUP-PAM | AGROSUP-DIJON | UP-SANTE | PAM | U974 | UPEC | IMRB | CNRS | UPEC-UPEM | UPMC | UNIV-BOURGOGNE | UNIV-PARIS5 | INSERM | SORBONNE-UNIVERSITE | UNIV-PARIS | SU-TI
Citation
Jordan Blondelle, Yusuke Ohno, Vincent Gache, Stéphane Guyot, Sébastien Storck, et al.. HACD1 , a regulator of membrane composition and fluidity, promotes myoblast fusion and skeletal muscle growth. Journal of molecular cell biology, Oxford UP, 2015, 7 (5), pp.429-440. ⟨10.1093/jmcb/mjv049⟩. ⟨hal-02290631⟩
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