Journal articles
Genotypic variability enhances the reproducibility of an ecological study
Alexandru Milcu
1, 2
Ruben Puga-Freitas
3
Aaron Ellison
4
Manuel Blouin
5
Stefan Scheu
6
Gregoire Freschet
2
Laura Rose
Sébastien Barot
3
Simone Cesarz
Nico Eisenhauer
7
Thomas Girin
8
Davide Assandri
Michael Bonkowski
9
Nina Buchmann
10
Olaf Butenschoen
11
Sebastien Devidal
1
Gerd Gleixner
12
Arthur Gessler
13
Agnès Gigon
3
Anna Greiner
Carlo Grignani
14
Amandine Hansart
15
Zachary Kayler
Markus Lange
Jean-Christophe Lata
3
Jean-François Le Galliard
15
Martin Lukac
16
Neringa Mannerheim
Marina Müller
Anne Pando
5
Paula Rotter
Michael Scherer-Lorenzen
17
Rahme Seyhun
Katherine Urban-Mead
Alexandra Weigelt
18
Laura Zavattaro
Jacques Roy
1
Alexandru Milcu 1, 2
Author IdHAL : alexandru-milcu ResearcherId : http://www.researcherid.com/rid/X-6267-2018 ORCID : https://orcid.org/0000-0002-2889-1234
Ruben Puga-Freitas 3
Author
Aaron M Ellison 4
Author
Manuel Blouin 5
Author Employer institution : AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement IdHAL : manuel-blouin ORCID : https://orcid.org/0000-0002-3928-9070
Simone Cesarz
Author
Nico Eisenhauer 7
Author
Thomas Girin 8
Author IdHAL : thomas-girin ORCID : https://orcid.org/0000-0002-5738-7731
Davide Assandri
Author
Michael Bonkowski 9
Author
Nina Buchmann 10
Author
Olaf Butenschoen 11
Author
Sebastien Devidal 1
Author
Jean-Christophe Lata 3
Author
Jean-François Le Galliard 15
Author IdHAL : jean-francois-le-galliard ResearcherId : http://www.researcherid.com/rid/E-8702-2011 ORCID : https://orcid.org/0000-0002-5965-9868
Martin Lukac 16
Author
Neringa Mannerheim
Author
Jacques Roy 1
Author
1
Écotron Européen de Montpellier - UPS 3248 (1 chemin de rioux
34980 Montferrier-sur-Lez - France)
2
CEFE - Centre d’Ecologie Fonctionnelle et Evolutive (1919 route de Mende - 34293 Montpellier cedex 5 - France)
- UPVM - Université Paul-Valéry - Montpellier 3 : UMR 5175 (Université Paul Valéry Montpellier 3 Route de Mende 34199 Montpellier Cedex 5 - France)
- INRA - Institut National de la Recherche Agronomique : UMR 5175 (France)
- Montpellier SupAgro - Centre international d'études supérieures en sciences agronomiques : UMR 5175 (2, place Pierre Viala - 34060 Montpellier Cedex 02 - France)
- EPHE - École pratique des hautes études : UMR 5175) (4-14 Rue Ferrus, 75014 Paris - France)
- PSL - Université Paris sciences et lettres (60 rue Mazarine 75006 Paris - France)
- UM - Université de Montpellier : UMR 5175 (163 rue Auguste Broussonnet - 34090 Montpellier - France)
- CNRS - Centre National de la Recherche Scientifique : UMR 5175 (France)
- IRD [France-Sud] - Institut de Recherche pour le Développement : UMR 5175 (911 avenue Agropolis, BP 64501 34394 Montpellier cedex 5 - France)
- Montpellier SupAgro - Institut national d’études supérieures agronomiques de Montpellier (2, place Viala 34060 Montpellier Cédex 2 - France)
3
IEES - Institut d'écologie et des sciences de l'environnement de Paris (UPMC, campus de Jussieu - Bât A - Paris (75005)
UPMC, campus de Jussieu - Tour 56/66 - Paris (75005)
AgroParisTech-INRA - Grignon (78)
INRA - Versailles (78)
IRD - Bondy (93)
U-PEC - Créteil (94)
- France)
- CNRS - Centre National de la Recherche Scientifique : UMR7618 (France)
- UPEC UP12 - Université Paris-Est Créteil Val-de-Marne - Paris 12 (61 avenue du Général de Gaulle - 94010 Créteil cedex - France)
- UPMC - Université Pierre et Marie Curie - Paris 6 : UM113 (4 place Jussieu - 75005 Paris - France)
- INRA - Institut National de la Recherche Agronomique : UMRA1392 (France)
4
Harvard University [Cambridge] (Massachusetts Hall, Cambridge, MA 02138 - United States)
5
Agroécologie [Dijon] (Agroécologie, AgroSup Dijon, INRAE, Univ. Bourgogne, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France - France)
- UB - Université de Bourgogne (Maison de l'université - Esplanade Érasme - BP 27877 - 21078 Dijon cedex - France)
- INRA - Institut National de la Recherche Agronomique : UMR INRA 1347 (France)
- UBFC - Université Bourgogne Franche-Comté [COMUE] (France)
- AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement : UMR1347 (26 Boulevard du Dr Petitjean - BP 87999 - 21079 Dijon cedex - France)
7
Department of Forest Resources (115 Green Hall, 1530 Cleveland Avenue N., St Paul, MN 55108 - United States)
- University of Minnesota [Twin Cities] (Minneapolis/St.Paul - United States)
- University of Minnesota System (United States)
8
IJPB - Institut Jean-Pierre Bourgin (INRA Centre de Versailles-Grignon, Route de St-Cyr (RD10), 78026 Versailles Cedex - France)
- INRA - Institut National de la Recherche Agronomique : UMR1318 (France)
- AgroParisTech (16 rue Claude Bernard F-75231 Paris Cedex 05 - France)
9
Department of Terrestrial Ecology, Institut of Zoology (Germany)
- University of Cologne ( Albertus-Magnus-Platz, 50923 Köln - Germany)
10
Institute of Plant, Animal and Agroecosystem Sciences (Switzerland)
11
J. F. Blumenbach Institute of Zoology and Anthropology (Berliner Str. 28, 37073 Göttingen, Germany - Germany)
- Georg-August-University [Göttingen] (Wilhelmsplatz 1, 37073 Göttingen - Germany)
12
MPI-BGC - Max Planck Institute for Biogeochemistry (Hans-Knoell-Str. 10, 07745 Jena
- Germany)
- Max-Planck-Gesellschaft (Jägerstrasse, 10-11, D-10117 Berlin - Germany)
13
Swiss Federal Research Institute (France)
14
Department of Agricultural, Forestry, and Food Sciences (Italy)
- University of Turin (Via Verdi, 8 - 10124 Turin - Italy)
15
CEREEP-Ecotron Ile de France (UMS 3194) (CNRS / ENS UMS 3194,
Centre de recherche en écologie expérimentale et prédictive - Ecotron Ile de France,
École Normale Supérieure, Rue du Château, 77140 St-Pierre-les-Nemours - France)
- CNRS - Centre National de la Recherche Scientifique : UMS3194 (France)
- Département de Biologie - ENS Paris (Département de Biologie
ENS Ulm
46 rue d 'Ulm
75005 Paris - France)
- ENS Paris - École normale supérieure - Paris (45, Rue d'Ulm - 75230 Paris cedex 05 - France)
- PSL - Université Paris sciences et lettres (60 rue Mazarine 75006 Paris - France)
- CNRS - Centre National de la Recherche Scientifique (France)
- INSERM - Institut National de la Santé et de la Recherche Médicale (101, rue de Tolbiac, 75013 Paris - France)
- ENS Paris - École normale supérieure - Paris (45, Rue d'Ulm - 75230 Paris cedex 05 - France)
16
School of Agriculture, Policy and Development, University of Reading (United Kingdom)
17
Faculty of Biology (Germany)
- University of Freiburg [Freiburg] (Friedrichstr. 39 79098 Freiburg - Germany)
18
Special Botany and Functional Biodiversity (Institute for Special Botany and Functional Biodiversity University of Leipzig 04103 Leipzig - Germany)
- Universität Leipzig [Leipzig] (Ritterstraße 26 04109 Leipzig - Germany)
Abstract : Many scientific disciplines are currently experiencing a 'reproducibility crisis' because numerous scientific findings cannot be repeated consistently. A novel but controversial hypothesis postulates that stringent levels of environmental and biotic standardization in experimental studies reduce reproducibility by amplifying the impacts of laboratory-specific environmental factors not accounted for in study designs. A corollary to this hypothesis is that a deliberate introduction of controlled systematic variability (CSV) in experimental designs may lead to increased reproducibility. To test this hypothesis, we had 14 European laboratories run a simple microcosm experiment using grass (Brachypodium distachyon L.) monocultures and grass and legume (Medicago truncatula Gaertn.) mixtures. Each laboratory introduced environmental and genotypic CSV within and among replicated microcosms established in either growth chambers (with stringent control of environmental conditions) or glasshouses (with more variable environmental conditions). The introduction of genotypic CSV led to 18% lower among-laboratory variability in growth chambers, indicating increased reproducibility, but had no significant effect in glasshouses where reproducibility was generally lower. Environmental CSV had little effect on reproducibility. Although there are multiple causes for the 'reproducibility crisis', deliberately including genetic variability may be a simple solution for increasing the reproducibility of ecological studies performed under stringently controlled environmental conditions.
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https://hal-agrosup-dijon.archives-ouvertes.fr/hal-01749308
Contributor : Administrateur Agrosupdijon <>
Submitted on : Thursday, March 29, 2018 - 12:12:30 PM
Last modification on : Wednesday, October 14, 2020 - 3:44:32 AM
Contributor : Administrateur Agrosupdijon <>
Submitted on : Thursday, March 29, 2018 - 12:12:30 PM
Last modification on : Wednesday, October 14, 2020 - 3:44:32 AM
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- HAL Id : hal-01749308, version 1
- DOI : 10.1038/s41559-017-0434-x
- PRODINRA : 428020
- PUBMED : 29335575
- WOS : 000426516400018
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Citation
Alexandru Milcu, Ruben Puga-Freitas, Aaron Ellison, Manuel Blouin, Stefan Scheu, et al.. Genotypic variability enhances the reproducibility of an ecological study. Nature Ecology & Evolution, Nature, 2018, 2 (2), pp.279 - 287. ⟨10.1038/s41559-017-0434-x⟩. ⟨hal-01749308⟩
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