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Uptake of UVc induced photoproducts of dipicolinic acid by Bacillus subtilis spores – Effects on the germination and UVc resistance of the spores

Jonathan Dikec 1 Maxime Pacheco 1 M. Lavaud 1, 2 Pascale Winckler 1, 2 Jean-Marie Perrier-Cornet 1, 2, * 
* Corresponding author
1 PMB - Procédés Microbiologiques et Biotechnologiques
PAM - Procédés Alimentaires et Microbiologiques [Dijon]
2 DImaCell - Dispositif Inter-régional d'Imagerie Cellulaire [Dijon]
PAM - Procédés Alimentaires et Microbiologiques, IBCT (ex IFR133) - Ingénierie et biologie cellulaire et tissulaire, Agroécologie [Dijon]
Abstract : Dipicolinic acid (DPA) is a specific molecule of bacterial spores which is essential to their resistance to various stresses such as ultraviolet (UV) exposure and to their germination. DPA has a particular photochemistry that remains imperfectly understood. In particular, due to its ability to absorb UVc radiation, it is likely to form in vitro a wide variety of photoproducts (DPAp) of which only about ten have been recently identified. The photochemical reactions resulting in DPAp, especially those inside the spores, are still poorly understood. Only one of these DPAp, which probably acts as a photosensitizer of DNA upon exposure to UVc, has been identified as having an impact on spores. However, as UVc is required to form DPAp, it is difficult to decouple the overall effect of UVc exposure from the possible effects of DPAp alone. In this study, DPAp were artificially introduced into the spores of the FB122 mutant strain of Bacillus subtilis, one that does not produce DPA. These experiments revealed that some DPAp may play a positive role for the spore. These benefits are visible in an improvement in spore germination rate and kinetics, as well as in an increase in their resistance to UVc exposure.
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https://hal-agrosup-dijon.archives-ouvertes.fr/hal-03792017
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Submitted on : Thursday, September 29, 2022 - 4:57:58 PM
Last modification on : Friday, December 2, 2022 - 12:59:17 PM

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Jonathan Dikec, Maxime Pacheco, M. Lavaud, Pascale Winckler, Jean-Marie Perrier-Cornet. Uptake of UVc induced photoproducts of dipicolinic acid by Bacillus subtilis spores – Effects on the germination and UVc resistance of the spores. Journal of Photochemistry and Photobiology B: Biology, 2022, 236, pp.112569. ⟨10.1016/j.jphotobiol.2022.112569⟩. ⟨hal-03792017⟩

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