Study and optimization of freeze-drying cycles of model probiotic: Morphology characterization of Lyophilisates by SEM - Institut Agro Dijon Accéder directement au contenu
Communication Dans Un Congrès Année : 2022

Study and optimization of freeze-drying cycles of model probiotic: Morphology characterization of Lyophilisates by SEM

Résumé

These experimental data are concerning the optimization of freeze-drying cycles of a model type Casei probiotic bacteria with water based formulations using lactose and polymer polyvinylpyrolidone (PVP) as cry/lyo protectants. After a previous study devoted to the influence of the main freeze-drying parameters (freezing rates; shelf temperature; total gas pressure) on the bacteria survival ratios, this paper reports some morphological data obtained by scanning electron microscopy (SEM) with the final lyophilisates. The numerous and original SEM images realized with different formulations of water binary or ternary mixtures with fixed operating conditions proved to be very useful to explain and to understand the different bacteria survival ratios observed., Thus, with bacteria or micro-organisms systems, this powerful investigation method of characterization of lyophilisates morphology should be more largely used in the future for setting up the optimal formulations parameters (composition) and the connected operating physical parameters of freezedrying steps (freezing rate, shelf temperature, total gas pressure).
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Dates et versions

hal-03773966 , version 1 (09-09-2022)

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Paternité - Pas d'utilisation commerciale - Pas de modification

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Séverine Crastes-Vessot, Ghania Degobert, Géraldine Agusti, Claudia Cogné, Julien Andrieu, et al.. Study and optimization of freeze-drying cycles of model probiotic: Morphology characterization of Lyophilisates by SEM. 22nd International Drying Symposium, Jun 2022, Worcester, United States. pp.1-9, ⟨10.55900/auksdudu⟩. ⟨hal-03773966⟩
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