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Effects of pulsed near infrared light (NIR) on Bacillus subtilis spores

Jonathan Dikec 1 Shaliha Bechoua 1 Pascale Winckler 2, 3 Jean-Marie Perrier-Cornet 2, 3 
2 PMB - Procédés Microbiologiques et Biotechnologiques
PAM - Procédés Alimentaires et Microbiologiques [Dijon]
3 DImaCell - Dispositif Inter-régional d'Imagerie Cellulaire [Dijon]
PAM - Procédés Alimentaires et Microbiologiques, IBCT (ex IFR133) - Ingénierie et biologie cellulaire et tissulaire, Agroécologie [Dijon], CSGA - Centre des Sciences du Goût et de l'Alimentation [Dijon]
Abstract : In this study, we develop a characterization of bacterial spore resistance to NIR pulsed light under modalities traditionally used in multiphoton microscopy. Energy dose and laser power are both key parameters in spore and bacterial cell inactivation. Surprisingly, spores and vegetative cells seem to show a similar sensitivity to pulsed NIR, spores being only 2-fold more resistant than their vegetative counterparts. This work enables us to eliminate certain hypotheses concerning the main driver of spore inactivation processes. Our findings suggest that damage leading to inactivation is mainly caused by photochemical reactions characterized by multiple possible pathways, including DNA damage or oxidation processes.
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Submitted on : Monday, August 29, 2022 - 8:52:59 AM
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Jonathan Dikec, Shaliha Bechoua, Pascale Winckler, Jean-Marie Perrier-Cornet. Effects of pulsed near infrared light (NIR) on Bacillus subtilis spores. Journal of Photochemistry and Photobiology B: Biology, Elsevier, 2022, 234, pp.112530. ⟨10.1016/j.jphotobiol.2022.112530⟩. ⟨hal-03762800⟩



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