Spectroscopic and microscopic characterization of dipicolinic acid and its salt photoproducts – A UVc effect study on DPA in solution and in bacterial spores - Institut Agro Dijon Accéder directement au contenu
Article Dans Une Revue Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy [1994-...] Année : 2022

Spectroscopic and microscopic characterization of dipicolinic acid and its salt photoproducts – A UVc effect study on DPA in solution and in bacterial spores

Résumé

Bacterial spores can cause significant problems such as food poisoning (like neurotoxin or emetic toxin) or serious illnesses (like anthrax or botulism). This dormant form of bacteria, made of several layers of barriers which provide extreme resistance to many abiotic stresses (radiation, temperature, pressure, etc.), are difficult to investigate in situ. To better understand the biological and chemical mechanisms involved and specific to spores resistance, the acquisition of environmental parameters is necessary. For that purpose, our research has been focused on the detection and analysis of a unique spore component, dipicolinic acid (DPA), used as the main in situ metabolite for sporulating bacteria detection. In its native form, DPA is only weakly fluorescent but after Ultraviolet irradiation at the wavelength of 254 nm (UVc), DPA photoproducts (DPAp) exhibit a remarkable fluorescence signal. These photoproducts are rarely identified and part of this study gives new insights offered by mass spectrometry (MS) in the determination of DPA photoproducts. Thanks to DPA assay techniques and fluorescence spectrometry, we highlighted the instability of photoproducts and introduced new assumptions on the effects of UVc on DPA. Studies in spectroscopy and microscopy allowed us to better understand these native probes in bacterial spores and will allow the implementation of a new method for studying the physico-chemical parameters of spore resistance.

Dates et versions

hal-03761812 , version 1 (26-08-2022)

Licence

Paternité - Pas d'utilisation commerciale - Pas de modification

Identifiants

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Maxime Pacheco, Jonathan Dikec, Pascale Winckler, Jean-Marie Perrier-Cornet, Christian Coelho. Spectroscopic and microscopic characterization of dipicolinic acid and its salt photoproducts – A UVc effect study on DPA in solution and in bacterial spores. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy [1994-..], 2022, 280, pp.121502. ⟨10.1016/j.saa.2022.121502⟩. ⟨hal-03761812⟩
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