Preparation and Characterization of Bioactive Chitosan Film Loaded with Cashew (Anacardium occidentale) Leaf Extract

Khursheed Ahmad Shiekh (1, 2) , Mooksupang Liangpanth (1) , Siriporn Luesuwan (1, 2) , Rinlanee Kraisitthisirintr (1, 2) , Kittaporn Ngiwngam (1) , Saroat Rawdkuen (1, 2) , Pornchai Rachtanapun (3) , Thomas Karbowiak (4) , Wirongrong Tongdeesoontorn (1, 2)

1 School of Agro-Industry [Mae Fah Luang University - Thaïlande]
2 MFU - Mae Fah Luang University [Thaïlande]
3 CMU - Chiang Mai University
4 PCAV - Physico-Chimie de l'Aliment et du Vin

Chitosan is a biopolymer known for its rapid biodegradability and film-forming properties. This research aimed to synthesize and characterize chitosan films loaded with cashew leaf extract (CLE) obtained from immature and mature cashew leaves via aqueous and 70% ethanolic extraction methods. Freeze-dried CLE samples were dissolved in 50% dimethyl sulfoxide for in vitro analysis and chitosan film preparation. The total phenolic content of mature cashew leaves extracted in ethanol (MECLE) showed higher free radicle scavenging activity by a 2,2-diphenyl-1-picrylhydrazyl assay than the other extracts (p < 0.05). MECLE displayed a lower minimal inhibitory concentration, minimum fungal concentration, and higher zone of inhibition against Aspergillus niger compared to the other treatments (p < 0.05). Film-forming solutions were prepared using 2% chitosan, 2% chitosan with 5% mature cashew leaves extracted in deionized water (MACLE) (w/v), and 2% chitosan with 5% MECLE (w/v), respectively, to cast films. Of these, 2% chitosan (CH) with 5% MECLE (CH-MECLE-5) displayed the highest thickness and water vapor transmission rate, water vapor permeability, and oxygen transmission rate when compared to other film samples (p < 0.05). The CH-MECLE-5 film showed the highest inhibition zone of A. niger compared to the control and treated films (p < 0.05). The lightness (L*) of the CH-MECLE-5 film decreased with increment in b* values, which represented the yellow color of the film. In addition, two-photon microscopy revealed a uniform distribution via the auto-fluorescent 3D structure of MECLE in the CH-MECLE-5 film. Therefore, chitosan combined with 5% MECLE may be a potential bioactive and eco-friendly packaging film.

Mots clés: Chitosan; Phytochemical extract; Gas barrier; Aspergillus niger; Antifungal film; Eco-friendly; Biopolymer film

Domaines: Biochimie, Biologie Moléculaire

Khursheed Ahmad Shiekh, Mooksupang Liangpanth, Siriporn Luesuwan, Rinlanee Kraisitthisirintr, Kittaporn Ngiwngam, et al.. Preparation and Characterization of Bioactive Chitosan Film Loaded with Cashew (Anacardium occidentale) Leaf Extract. Polymers, 2022, 14 (3), pp.540. ⟨10.3390/polym14030540⟩. ⟨hal-03578852⟩