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Poster De Conférence Année : 2019

Adaptive evolution to improve acid tolerance in Oenococcus oeni

Résumé

Oenococcus oeni is a lactic acid bacterium (LAB) mainly responsible for the malolactic fermentation (MLF) in wine. MLF plays an important role in determining the final quality of wines [1]. Even though this LAB is naturally present in musts, wines and oenological environment, spontaneous MLF are usually unpredictable because of the stressful conditions and especially due to acidity [2]. The consequence of the mismanagement of this step might lead to the depreciation of wine quality. To obtain a clone more tolerant to acidity, we undertook a replication of O. oeni until 450 generations in a temporally varying environment (pH 5.3 to 2.9) to improve acid tolerance. To discriminate stress tolerance of evolved populations versus parental strain an acid stress was performed to both population.

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Dates et versions

hal-03150729 , version 1 (24-02-2021)

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  • HAL Id : hal-03150729 , version 1

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Frédérique Julliat, N. Tourti, Stéphane Guyot, Herve Alexandre, Cosette Grandvalet. Adaptive evolution to improve acid tolerance in Oenococcus oeni. 11th international synposium of oenology of Bordeaux, Jun 2019, Bordeaux, France. ⟨hal-03150729⟩
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