Skip to Main content Skip to Navigation
Journal articles

Functional genomics of Lactobacillus casei establishment in the gut

Abstract : Although the composition of the gut microbiota and its symbiotic contribution to key host physiological functions are well established, little is known as yet about the bacterial factors that account for this symbiosis. We selected Lactobacillus casei as a model microorganism to proceed to genomewide identification of the functions required for a symbiont to establish colonization in the gut. As a result of our recent development of a transposon-mutagenesis tool that overcomes the barrier that had prevented L. casei random mutagenesis, we developed a signature-tagged mutagenesis approach combining whole-genome reverse genetics using a set of tagged transposons and in vivo screening using the rabbit ligated ileal loop model. After sequencing transposon insertion sites in 9,250 random mutants, we assembled a library of 1,110 independent mutants, all disrupted in a different gene, that provides a representative view of the L. casei genome. By determining the relative quantity of each of the 1,110 mutants before and after the in vivo challenge, we identified a core of 47 L. casei genes necessary for its establishment in the gut. They are involved in housekeeping functions, metabolism (sugar, amino acids), cell wall biogenesis, and adaptation to environment. Hence we provide what is, to our knowledge, the first global functional genomics analysis of L. casei symbiosis.
Document type :
Journal articles
Complete list of metadatas

https://hal-agrosup-dijon.archives-ouvertes.fr/hal-02953377
Contributor : Administrateur Agrosupdijon <>
Submitted on : Wednesday, September 30, 2020 - 10:20:20 AM
Last modification on : Thursday, October 1, 2020 - 3:32:40 AM

Links full text

Identifiers

Citation

Helene Licandro-Seraut, Helene Scornec, Thierry Pédron, Jean-François Cavin, Philippe Sansonetti. Functional genomics of Lactobacillus casei establishment in the gut. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2014, 111 (30), pp.E3101-E3109. ⟨10.1073/pnas.1411883111⟩. ⟨hal-02953377⟩

Share

Metrics

Record views

18