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Modeling hexanal production in oxido-reducing conditions by the yeast Yarrowia lipolytica

Abstract : Hexanal produced by cells of a recombinant Yarrowia lipolytica yeast expressing the hydroperoxide lyase (HPL) from green bell pepper fruit was studied under oxido-reducing conditions using the reducing dithiotreitol and oxidizing potassium ferricyanide compounds. The combined effect of pH, linoleic acid 13-hydroperoxides concentration, temperature and oxido-reducing molecules on the hexanal production was studied. Significant positive effects for the hexanal production were found using high concentrations of hydroperoxides (100 mM, 30 g/L). Adding reducing molecules enhanced significantly hexanal production while the oxidizing molecules had an inhibitory effect. Combined effects of 13-hydroperoxides and dithiotreitol were optimised by a central composite design and a model was proposed. Finally, 6 mM (600 mg/L) of hexanal was obtained when 119 mM of 13-hydroperoxides (37 g/L) and 50 mM of dithiotreitol were introduced directly in the biocatalytic medium of the yeast Y. lipolytica.
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https://hal-agrosup-dijon.archives-ouvertes.fr/hal-02467733
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Submitted on : Wednesday, February 5, 2020 - 11:18:16 AM
Last modification on : Thursday, June 18, 2020 - 12:32:06 PM

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Mirna Patricia Santiago-Gómez, Hoa Tran Thanh, Joëlle de Coninck, Rémy Cachon, Selim Kermasha, et al.. Modeling hexanal production in oxido-reducing conditions by the yeast Yarrowia lipolytica. Process Biochemistry, Elsevier, 2009, 44 (9), pp.1013-1018. ⟨10.1016/j.procbio.2009.04.028⟩. ⟨hal-02467733⟩

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