 |
Journal articles
Monitoring of transglutaminase crosslinking reaction by 1H NMR spectroscopy on model substrates
Abstract : A new method based on 1H NMR spectroscopy was developed for monitoring transglutaminase crosslinking reaction with model molecules (CBZ-Gln-Gly and N-α-acetyl-lysine). The transglutaminase reaction led to the appearance of new resonances on NMR spectrum as well as significant decrease in others. The new observed resonances, originated from newly formed ɛ-(γ-glutamyl)lysine isopeptide bonds, evidence the enzymatic reaction and allow to quantify the ɛ-(γ-glutamyl)lysine fragment. Moreover, the decrease in resonance intensity, originated from lysine, permit to determine the crosslinking degree. These results obtained by 1H NMR spectroscopy can be used as an alternative method to LC–MS, reducing drastically the analysis time from 7 days to 2 h.
https://hal-agrosup-dijon.archives-ouvertes.fr/hal-02413068
Contributor : Admin Agrosupdijon Connect in order to contact the contributor
Submitted on : Monday, December 16, 2019 - 9:43:12 AM
Last modification on : Thursday, December 16, 2021 - 3:55:49 AM
Contributor : Admin Agrosupdijon Connect in order to contact the contributor
Submitted on : Monday, December 16, 2019 - 9:43:12 AM
Last modification on : Thursday, December 16, 2021 - 3:55:49 AM