Journal articles
Structural studies of adsorbed protein (betalactoglobulin) on natural clay (montmorillonite)
Ali Assifaoui
1, *
Lucie Huault
1
Cyrielle Maissiat
1
Chloé Roullier-Gall
1
Philippe Jeandet
2
Jerôme Hirschinger
3
Jesus Raya
3
Maguy Jaber
4
Jean-François Lambert
5
Philippe Cayot
1
Régis Gougeon
1
Camille Loupiac
1
*
Corresponding author
Ali Assifaoui 1
Correspondent author
Lucie Huault 1
Author
Cyrielle Maissiat 1
Author
Chloé Roullier-Gall 1
Author
Jean-François Lambert 5
Author
Philippe Cayot 1
Author Employer institution : AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement IdHAL : philippe-cayot IdRef : https://www.idref.fr/035364130 ORCID : https://orcid.org/0000-0002-6998-8569
Régis Gougeon 1
Author
Camille Loupiac 1
Author IdHAL : camille-loupiac IdRef : https://www.idref.fr/165623918 ORCID : https://orcid.org/0000-0003-2343-0313
1
PAPC - Procédés Alimentaires et Physico-Chimie (Université de Bourgogne - 1 esplanade Erasme - 21000 Dijon - France)
- PAM - Procédés Alimentaires et Microbiologiques (Université de Bourgogne - 1 esplanade Erasme - 21000 Dijon - France)
- UB - Université de Bourgogne (Maison de l'université - Esplanade Érasme - BP 27877 - 21078 Dijon cedex - France)
- AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement : UMR MA AGROSUP 2012.02.102 (26 Boulevard du Dr Petitjean - BP 87999 - 21079 Dijon cedex - France)
2
SDRP - LABORATOIRE DE STRESS, DÉFENSES ET REPRODUCTION DES PLANTES (Université de Reims Champagne-Ardenne UFR des Sciences Exactes et Naturelles Bâtiment 18 Moulin de la Housse - BP 1039 51687 REIMS Cedex 2 - FRANCE - France)
- URCA - Université de Reims Champagne-Ardenne (9 boulevard de la Paix - CS 60005 - 51724 Reims cedex - France)
3
Institut de Chimie de Strasbourg (1 rue Blaise Pascal BP 296R8 67008 STRASBOURG CEDEX - France)
- UNISTRA - Université de Strasbourg (4 rue Blaise Pascal - CS 90032 - 67081 Strasbourg cedex - France)
- CNRS - Centre National de la Recherche Scientifique : UMR7177 (France)
4
LAMS - Laboratoire d'Archéologie Moléculaire et Structurale (Sorbonne-Université 4 Place Jussieu 75252 Paris Cedex 5 - France)
- SU - Sorbonne Université (21 rue de l’École de médecine - 75006 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR8220 (France)
5
LRS - Laboratoire de Réactivité de Surface (Tour 54- 2ème etg 4 Place Jussieu - Case 178 75252 PARIS CEDEX 05 - France)
- SU - Sorbonne Université (21 rue de l’École de médecine - 75006 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR7197 (France)
Abstract : In this work, the adsorption of a small globular protein (betalactoglobulin, BLG), on a natural montmorillonite clay (Mt) was investigated in acidic buffer (pH = 3). The combination of different characterization techniques such as zetametry, X-ray diffraction, transmission electronic microscopy, fluorescence and solid state nuclear magnetic resonance spectroscopies shed light on the interaction mechanism between the clay mineral and the proteins. For low BLG concentration, a slight increase of the interlayer spacing of the clay mineral was noticed as well as structural changes of the protein. In contrast, as the concentration of BLG increased, the adsorption led to a partial exfoliation of the clay mineral, accompanied with significant secondary structural changes of the protein characterized by a loss of β-sheet organization. Altogether, our results revealed an unexpected adsorption scheme where the increase of the BLG/Mt weight ratio of the hybrid material leads to a partial exfoliation of the Mt, but at the expense of the protein native structure.
Document type :
Journal articles
Complete list of metadatas
https://hal-agrosup-dijon.archives-ouvertes.fr/hal-02358967
Contributor : Administrateur Agrosupdijon <>
Submitted on : Tuesday, November 12, 2019 - 11:32:47 AM
Last modification on : Wednesday, July 1, 2020 - 3:26:02 PM
Contributor : Administrateur Agrosupdijon <>
Submitted on : Tuesday, November 12, 2019 - 11:32:47 AM
Last modification on : Wednesday, July 1, 2020 - 3:26:02 PM
Identifiers
- HAL Id : hal-02358967, version 1
- DOI : 10.1039/C4RA11607K
Collections
AGROSUP-DIJON | AGROSUP-PAM | URCA | UNIV-BOURGOGNE | CNRS | INC-CNRS | LAMS | PAM | SITE-ALSACE | SORBONNE-UNIVERSITE | SU-SCIENCES
Citation
Ali Assifaoui, Lucie Huault, Cyrielle Maissiat, Chloé Roullier-Gall, Philippe Jeandet, et al.. Structural studies of adsorbed protein (betalactoglobulin) on natural clay (montmorillonite). RSC Advances, Royal Society of Chemistry, 2014, 4 (105), pp.61096-61103. ⟨10.1039/C4RA11607K⟩. ⟨hal-02358967⟩
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