Skip to Main content Skip to Navigation
New interface
Journal articles

Time-resolved Fluorescence and Generalized Polarization: Innovative tools to assess bull sperm membrane dynamics during slow freezing

Shaliha Bechoua 1, 2 Pascale Winckler 3 Audrey Jossier 1 Caroline Peltier 1 Frédéric Delize 4 Noémie Devaux 4 Jean-Marie Perrier-Cornet 5, 6 Hélène Simonin 5 
3 DImaCell - Dispositif Inter-régional d'Imagerie Cellulaire [Dijon]
PAM - Procédés Alimentaires et Microbiologiques, IBCT (ex IFR133) - Ingénierie et biologie cellulaire et tissulaire, Agroécologie [Dijon]
5 PMB - Procédés Microbiologiques et Biotechnologiques
PAM - Procédés Alimentaires et Microbiologiques, PAM - Procédés Alimentaires et Microbiologiques [Dijon]
Abstract : During slow freezing, spermatozoa undergo membrane alterations that compromise their ability of fertilizing. These alterations are cause either by cold shock or by the use of cryoprotectants known to be cytotoxic. However, little is known about the membrane changes that occurred during freezing. Here, we combined Generalized Polarization (GP), Time-resolved Fluorescence and laurdan fluorescence properties to investigate the changes in membrane fluidity and dynamics during slow freezing of bull sperm. We successfully demonstrated that laurdan may be distributed in three different local environments that correspond to different membrane lipid composition. These environments wont behave the same way when the cells will be subjected to either a chemical treatment (adding the cryoprotectants) or a physical treatment (freezing).
Document type :
Journal articles
Complete list of metadata

https://hal-agrosup-dijon.archives-ouvertes.fr/hal-02354329
Contributor : Accord Elsevier CCSD Connect in order to contact the contributor
Submitted on : Tuesday, December 21, 2021 - 1:40:17 PM
Last modification on : Friday, December 2, 2022 - 1:00:22 PM
Long-term archiving on: : Wednesday, March 23, 2022 - 10:20:13 AM

File

S0011224019301166.pdf
Files produced by the author(s)

Licence


Distributed under a Creative Commons Attribution - NonCommercial 4.0 International License

Identifiers

Citation

Shaliha Bechoua, Pascale Winckler, Audrey Jossier, Caroline Peltier, Frédéric Delize, et al.. Time-resolved Fluorescence and Generalized Polarization: Innovative tools to assess bull sperm membrane dynamics during slow freezing. Cryobiology, 2019, 91, pp.69-76. ⟨10.1016/j.cryobiol.2019.10.196⟩. ⟨hal-02354329⟩

Share

Metrics

Record views

67

Files downloads

46