In vitro and in silico hormonal activity studies of di-(2-ethylhexyl)terephthalate, a di-(2-ethylhexyl)phthalate substitute used in medical devices, and its metabolites

Nicolas Kambia 1 Isabelle Séverin 2 Amaury Farce 1 Emmanuel Moreau 3 Laurence Dahbi 2 Claire Duval 1 Thierry Dine 1 Valérie Sautou 4, 5 Marie-Christine Chagnon 2
1 GRITA - Groupe de Recherche sur les formes Injectables et les Technologies Associées (GRITA)-EA 7365
2 NUTOX - Equipe NuTox (LNC - U1231)
LNC - Lipides - Nutrition - Cancer [Dijon - U1231]
3 CHU Clermont-Ferrand
4 ICCF - Institut de Chimie de Clermont-Ferrand - Clermont Auvergne
5 CHU Clermont-Ferrand, Pôle Pharmacie
Abstract : Plasticizers added to polyvinylchloride used in medical devices can be released into patients’ biological fluids. The substitution of di‐(2‐ethylhexyl)phthalate (DEHP) by alternative plasticizers is essential but their safety must be demonstrated. DEHP, di‐(2‐ethylhexyl)terephthalate (DEHT) and their metabolites were investigated using level 2 Organization for Economic Co‐operation and Development bioassays to screen for in vitro hormonal changes. Differences between the DEHP and DEHT metabolites were observed. Albeit weak, the hormonal activities of DEHT‐derived metabolites, e.g., 5‐OH metabolite of mono‐(ethylhexyl)terephthalate (5‐OH‐MEHT), were detected and the results of docking experiments performed on estrogen receptor alpha and androgen receptor agreed with the biological results. A co‐stimulation of human estrogen receptor alpha and human androgen receptor was also observed. With regard to steroidogenesis, a 16‐fold increase in estrogen synthesis was measured with 5‐OH‐MEHT. Therefore, even if DEHT remains an interesting alternative to DEHP because of its low migration from medical devices, it seems important to verify that multi‐exposed patients in neonatal intensive care units do not have urinary levels of oxidized metabolites, in particular 5‐OH‐MEHT, suggesting a potential endocrine‐disrupting effect.
Keywords : DEHP H295R steroidogenesis assay Docking DEHT testosterone screen human androgen or estrogen receptor
Document type :
Journal articles
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https://hal-agrosup-dijon.archives-ouvertes.fr/hal-02068658
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Submitted on : Friday, March 15, 2019 - 10:38:21 AM
Last modification on : Friday, November 8, 2019 - 10:55:38 AM

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AGROSUP-DIJON | AGROSUP-LNC | UNIV-BOURGOGNE | LNC-UMR866 | LNC-NUTOX | PRES_CLERMONT | UNIV-LILLE | ICC | SIGMA-CLERMONT | INC-CNRS

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Nicolas Kambia, Isabelle Séverin, Amaury Farce, Emmanuel Moreau, Laurence Dahbi, et al.. In vitro and in silico hormonal activity studies of di-(2-ethylhexyl)terephthalate, a di-(2-ethylhexyl)phthalate substitute used in medical devices, and its metabolites. Journal of Applied Toxicology, Wiley, 2019, 39, pp.1043-1056. ⟨10.1002/jat.3792⟩. ⟨hal-02068658⟩

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