Nitrogen modulation of Medicago truncatula resistance to Aphanomyces euteiches depends on plant genotype

Elise Thalineau 1 Carine Fournier 1 Antoine Gravot 2 David Wendehenne 1 Sylvain Jeandroz 1 Hoai-Nam Truong 1
1 Agroécologie [Dijon]
2 IGEPP - Institut de Génétique, Environnement et Protection des Plantes
Abstract : Nitrogen (N) availability can impact plant resistance to pathogens by regulating plant immunity. To better understand the links between N nutrition and plant defence, we analysed the impact of N availability on Medicago truncatula resistance to the root pathogen Aphanomyces euteiches. This oomycete is considered as the most limiting factor for legume production. Ten plant genotypes were tested in vitro for their resistance to A. euteiches in either complete or N-deficient medium. N-deficiency led to enhanced or reduced susceptibility depending on plant genotype. Focusing on four genotypes displaying contrasted responses we determined the impact of N-deficiency on plant growth and shoot N concentration and performed expression analyses on N- and defence-related genes as well as quantification of soluble phenolics and of root contents in different amino-acids. Our analyses suggest that N modulation of plant resistance is not linked to plant response to N deprivation nor to mechanisms previously identified to be involved in plant resistance. Furthermore our studies highlight a role of glutamine in mediating susceptibility to A. euteiches in M. truncatula.
Keywords : Amino acids Aphanomyces euteiches Glutamine Medicago truncatula nitrate nitrogen plant defence
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Journal articles
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Submitted on : Tuesday, February 5, 2019 - 4:12:42 PM
Last modification on : Monday, February 11, 2019 - 3:16:01 PM

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AGROSUP-DIJON | AGROSUP-AE | INRA | UNAM | UNIV-BOURGOGNE | AGROECOLOGIE | BIOSIT | UR1-UFR-SVE | UNIV-RENNES1 | UNIV-RENNES

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Elise Thalineau, Carine Fournier, Antoine Gravot, David Wendehenne, Sylvain Jeandroz, et al.. Nitrogen modulation of Medicago truncatula resistance to Aphanomyces euteiches depends on plant genotype. Molecular Plant Pathology, Wiley, 2018, 19 (3), pp.664-676. ⟨10.1111/mpp.12550⟩. ⟨hal-02008341⟩

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