Soil biodiversity as a major driver for the control of populations of the pathogenic bacterium Listeria monocytogenes in soil

Abstract : Disease regulation is one of the many services that ecosystems provide. Agroecosystems are dedicated to the provision of high quality, safe food and sustainability is a major issue. Biological contamination of the food supply may raise food safety issues. Prevention of contamination of the food supply requires a thorough understanding of the ecology of pathogenic microorganisms. Listeria monocytogenes is a food pathogen found in many habitats including soil, plants, water systems and food processing factories. We designed microcosm experiments to decipher extrinsic factors that drive the fate of this model of Human pathogen in soil. We analysed population dynamics over 80 days after inoculation of a large set of 100 soils selected from the French Réseau de la Qualité des Sols (RMQS), a country-wide venture of monitoring of the quality of soils in France. The population of L. monocytogenes decreased over time but the survival ratio depended on the soil under scrutiny. Detailed information such as soil characteristics and land use were integrated into an analysis of variance. Chemical properties, especially the basic cation saturation ratio and soil texture were factors affecting survival of L. monocytogenes. Analysis of the results collected under similar experimental conditions in the same set of soils but sterilised by ionisation indicated that the endogenous soil microbiota had a major impact on the decrease of the population of L. monocytogenes. This prompted us to investigate the relationship between the biodiversity of the soil microbiota and the inhibition of the population of L. monocytogenes. By a dilution to extinction approach, we investigated the consequences of soil diversity erosion on the fate of a human bacterial pathogen in the telluric environment. Diversity analysis showed that both soil diversity and phylogenetic composition affected survival of L. monocytogenes. Overall, our results demonstrate that soil biodiversity is a major driver that limit invasion of soil by pathogenic bacteria.Conclusions Depending on the incubation conditions, the fitness of the deletion mutants were affected. During its saprophytic life in soil habitat, L. monocytogenes have to cope with ever-changing environmental conditions and adapt in order to sustain life. Integration of various stimuli results in a coordinated response including communication and stress response systems through AgrA- and σB-mediated regulation.Conclusions The fate of L. monocytogenes is dependent on both abiotic and biotic characteristics and the latter have a major impact on the dynamics of the populations of L. monocytogenes in soil. Major transcriptome reshaping was observed where L. monocytogenes recruits its repertoire of transporters and specific pathways to access and utilise the available substrates. The biotic environment further affects transcriptome and triggers further regulation.
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Submitted on : Wednesday, January 30, 2019 - 9:43:47 AM
Last modification on : Thursday, January 31, 2019 - 1:20:43 AM

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  • HAL Id : hal-01999345, version 1
  • PRODINRA : 282575

Citation

Pascal Piveteau, Anne-Laure Vivant, Dominique Garmyn, Aude Locatelli, Laurent Gal, et al.. Soil biodiversity as a major driver for the control of populations of the pathogenic bacterium Listeria monocytogenes in soil. 1. Global Soil Biodiversity Initiative conference (GSBI), Dec 2014, Dijon, France. pp.617, Assessing soil biodiversity and its role for ecosystem services. ⟨hal-01999345⟩

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