9-cis-Retinoic acid enhances fatty acid-induced expression of the liver fatty acid-binding protein gene

Abstract : The role of retinoic acids (RA) on liver fatty acid-binding protein (L-FABP) expression was investigated in the well differentiated FAO rat hepatoma cell line. 9-cis-Retinoic acid (9-cis-RA) specifically enhanced L-FABP mRNA levels in a time- and dose-dependent manner. The higher induction was found 6 h after addition of 10(-6) M 9-cis-RA in the medium. RA also enhanced further both L-FABP mRNA levels and cytosolic L-FABP protein content induced by oleic acid. The retinoid X receptor (RXR) and the peroxisome proliferator-activated receptor (PPAR), which are known to be activated, respectively, by 9-cis-RA and long chain fatty acid (LCFA), co-operated to bind specifically the peroxisome proliferator-responsive element (PPRE) found upstream of the L-FABP gene. Our result suggest that the PPAR-RXR complex is the molecular target by which 9-cis-RA and LCFA regulate the L-FABP gene.
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Journal articles
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Submitted on : Friday, November 9, 2018 - 11:36:23 AM
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Hélène Poirier, Olivier Braissant, Isabelle Niot, W. Wahli, Philippe Besnard. 9-cis-Retinoic acid enhances fatty acid-induced expression of the liver fatty acid-binding protein gene. FEBS Letters, Wiley, 1997, 412 (3), pp.480 - 484. ⟨10.1016/S0014-5793(97)00830-2⟩. ⟨hal-01917245⟩

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