Tube-Gel: A Fast and Effective Sample Preparation Method for High-Throughput Quantitative Proteomics

Leslie Muller (1) , Luc Fornecker (1) , Sarah Cianférani (1) , Christine Carapito (1)

1 IPHC - Institut Pluridisciplinaire Hubert Curien

Sample preparation is a key step in proteomics workflows. Tube-gel (TG) is a fast and repeatable sample preparation method that consists in the instantaneous trapping of the sample in a polyacrylamide gel matrix. It takes advantage of in-gel sample preparations by allowing the use of high concentrations of sodium-dodecyl sulfate but avoids the time-consuming step of electrophoresis. Therefore, TG limits the sample handling and is thus particularly suitable for high-throughput quantitative proteomics when large sample numbers have to be processed, as it is often the case in biomarker research and clinical proteomics projects.

Mots clés: Tube-gel; sample preparation; high-throughput; detergent-compatibility; quantitative proteomics

Domaines: Chimie

Leslie Muller, Luc Fornecker, Sarah Cianférani, Christine Carapito. Tube-Gel: A Fast and Effective Sample Preparation Method for High-Throughput Quantitative Proteomics. Proteomics for biomarker discovery, pp.123-127, 2019, ⟨10.1007/978-1-4939-9164-8_8⟩. ⟨hal-02341533⟩